Journal article

Long-term single-cell imaging and simulations of microtubules reveal principles behind wall patterning during proto-xylem development

Rene Schneider, Kris van't Klooster, Kelsey L Picard, Jasper van der Gucht, Taku Demura, Marcel Janson, Arun Sampathkumar, Eva E Deinum, Tijs Ketelaar, Staffan Persson

NATURE COMMUNICATIONS | NATURE RESEARCH | Published : 2021

Abstract

Plants are the tallest organisms on Earth; a feature sustained by solute-transporting xylem vessels in the plant vasculature. The xylem vessels are supported by strong cell walls that are assembled in intricate patterns. Cortical microtubules direct wall deposition and need to rapidly re-organize during xylem cell development. Here, we establish long-term live-cell imaging of single Arabidopsis cells undergoing proto-xylem trans-differentiation, resulting in spiral wall patterns, to understand microtubule re-organization. We find that the re-organization requires local microtubule de-stabilization in band-interspersing gaps. Using microtubule simulations, we recapitulate the process in silic..

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University of Melbourne Researchers

Grants

Awarded by SRON space research programme - Netherlands Organization for Scientific Research (NWO)


Awarded by ARC


Awarded by Villum Investigator grant


Awarded by Novo Nordisk Laureate grant


Funding Acknowledgements

K.v.t.K. was supported by the SRON space research programme with project number GO-MG/15, which is financed by the Netherlands Organization for Scientific Research (NWO). We thank the Wageningen Light Microscopy Centre (WU) and the Biological Optical Microscopy Platform (BOMP, University of Melbourne) for the use of their facilities, and Bela Mulder for helpful discussions. S.P. was supported by R@MAP Professor Funds at University of Melbourne and an ARC DP and FT grants (DP190101941; FT160100218), and a Villum Investigator grant (Project ID: 25915) and Novo Nordisk Laureate grant (NNF19OC0056076).