Dynamic Changes to the Skeletal Muscle Proteome and Ubiquitinome Induced by the E3 Ligase, ASB2 beta
Craig A Goodman, Jonathan R Davey, Adam Hagg, Benjamin L Parker, Paul Gregorevic
MOLECULAR & CELLULAR PROTEOMICS | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2021
Ubiquitination is a posttranslational protein modification that has been shown to have a range of effects, including regulation of protein function, interaction, localization, and degradation. We have previously shown that the muscle-specific ubiquitin E3 ligase, ASB2β, is downregulated in models of muscle growth and that overexpression ASB2β is sufficient to induce muscle atrophy. To gain insight into the effects of increased ASB2β expression on skeletal muscle mass and function, we used liquid chromatography coupled to tandem mass spectrometry to investigate ASB2β-mediated changes to the skeletal muscle proteome and ubiquitinome, via a parallel analysis of remnant diGly-modified peptides. ..View full abstract
Related Projects (3)
USING GENE DELIVERY TECHNOLOGIES TO DEFINE NOVEL MECHANISMS OF SKELETAL MUSCLE ADAPTATION, AND DEVELOP MUSCLE-DIRECTED INTERVENTIONS FOR FRAILTY AND SERIOUS ILLNESS
The focus of my research is to investigate the cellular mechanisms underlying regulation of skeletal muscle size and function in health and ..
Awarded by National Health and Medical Research Council of Australia (NHMRC)
Awarded by Early Career Fellowship (NHMRC, Australia)
Awarded by Senior Research Fellowship (NHMRC, Australia)
Studies were supported by project grants (1121500, 1156562) from the National Health and Medical Research Council of Australia (NHMRC), an Early Career Fellowship (1072129, NHMRC, Australia) and Driving Research Momentum scheme (The University of Melbourne) awarded to B. L. P., and a Senior Research Fellowship (1117835, NHMRC, Australia) awarded to P. G. A. H. was supported by an Australian Post Graduate Award (Department of Education and Training, Australian Government) and a PhD stipend top-up award from the Baker Heart and Diabetes Institute Bright Sparks program.