Synthetic B-Cell Epitopes Eliciting Cross-Neutralizing Antibodies: Strategies for Future Dengue Vaccine
Babu Ramanathan, Chit Laa Poh, Kristin Kirk, William John Hannan McBride, John Aaskov, Lara Grollo
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2016
Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vacci..View full abstract
Awarded by Sunway University, Kuala Lumpur, Malaysia
The work was supported by Swinburne University of Technology: Researcher Development Scheme, Melbourne, Australia, and Grollo Ruzzene Foundation Research Grant. Babu Ramanathan was supported by an International Post-graduate Research Scholarship (IPRS). Support of a Research Fellowship to Babu Ramanathan and funding from internal research grant (INT-VCO-2016-01) from Sunway University, Kuala Lumpur, Malaysia is gratefully acknowledged.