Arabidopsis REI-LIKE proteins activate ribosome biogenesis during cold acclimation
Bo Eng Cheong, Olga Beine-Golovchuk, Michal Gorka, William Wing Ho Ho, Federico Martinez-Seidel, Alexandre Augusto Pereira Firmino, Aleksandra Skirycz, Ute Roessner, Joachim Kopka
SCIENTIFIC REPORTS | NATURE RESEARCH | Published : 2021
Arabidopsis REIL proteins are cytosolic ribosomal 60S-biogenesis factors. After shift to 10 °C, reil mutants deplete and slowly replenish non-translating eukaryotic ribosome complexes of root tissue, while controlling the balance of non-translating 40S- and 60S-subunits. Reil mutations respond by hyper-accumulation of non-translating subunits at steady-state temperature; after cold-shift, a KCl-sensitive 80S sub-fraction remains depleted. We infer that Arabidopsis may buffer fluctuating translation by pre-existing non-translating ribosomes before de novo synthesis meets temperature-induced demands. Reil1 reil2 double mutants accumulate 43S-preinitiation and pre-60S-maturation complexes and a..View full abstract
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