Journal article

Role of nicotinic acetylcholine receptor subunits in the mode of action of neonicotinoid, sulfoximine and spinosyn insecticides in Drosophila melanogaster

Trent Perry, Wei Chen, Razi Ghazali, Ying Ting Yang, Danielle Christesen, Felipe Martelli, Chris Lumb, Hang Ngoc Bao Luong, Judith Mitchell, Jessica K Holien, Michael W Parker, Thomas C Sparks, Philip Batterham



Insecticides remain valuable tools for the control of insect pests that significantly impact human health and agriculture. A deeper understanding of insecticide targets is important in maintaining this control over pests. Our study systematically investigates the nicotinic acetylcholine receptor (nAChR) gene family, in order to identify the receptor subunits critical to the insect response to insecticides from three distinct chemical classes (neonicotinoids, spinosyns and sulfoximines). Applying the CRISPR/Cas9 gene editing technology in D. melanogaster, we were able to generate and maintain homozygous mutants for eight nAChR subunit genes. A ninth gene (Dβ1) was investigated using somatic C..

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Awarded by Australian Research Council

Funding Acknowledgements

We thank Mr. Gerald Watson and Dr. Melissa Siebert (Corteva Agriscience) for useful discussions and feedback during the preparation of this article. Dow AgroSciences synthesised sulfoxaflor and provided funding assistance for sulfoxaflor studies. Fly strains were sourced from the Bloomington Drosophila Stock Centre and the Australian Drosophila Biomedical Research Support Facility provided quarantine facilities for imported fly strains. Funding for this research was provided through an Australian Research Council Discovery Project awarded to P.B. (DP160100332). M.W.P. is a National Health and Medical Research Council Senior Principal Research Fellow and J.K.H. was a Cure Cancer Australia/Leukaemia Foundation Postdoctoral Fellow during these studies and is now a RMIT Vice Chancellor's Research Fellow. Infrastructure support from the NHMRC Independent Research Institutes Infrastructure Support Scheme and the Victorian State Government Operational Infrastructure Support Program to St Vincent's Institute is gratefully acknowledged.