Journal article

A Pipeline towards the Biochemical Characterization of the Arabidopsis GT14 Family

Lingling Xuan, Jie Zhang, Weitai Lu, Pawel Gluza, Berit Ebert, Toshihisa Kotake, Mengzhu Lu, Yuan Zhang, Mads H Clausen, Kim L Johnson, Monika S Doblin, Joshua L Heazlewood, Antony Bacic, Lili Song, Wei Zeng



Glycosyltransferases (GTs) catalyze the synthesis of glycosidic linkages and are essential in the biosynthesis of glycans, glycoconjugates (glycolipids and glycoproteins), and glycosides. Plant genomes generally encode many more GTs than animal genomes due to the synthesis of a cell wall and a wide variety of glycosylated secondary metabolites. The Arabidopsis thaliana genome is predicted to encode over 573 GTs that are currently classified into 42 diverse families. The biochemical functions of most of these GTs are still unknown. In this study, we updated the JBEI Arabidopsis GT clone collection by cloning an additional 105 GT cDNAs, 508 in total (89%), into Gateway-compatible vectors for d..

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Awarded by National Natural Science Foundation of China

Awarded by Overseas Expertise Introduction Project for Discipline Innovation (111 Project)

Awarded by Australia Research Council

Awarded by Novo Nordisk Foundation

Awarded by Carlsberg Foundation

Funding Acknowledgements

This research was funded by the National Natural Science Foundation of China (grant no. 31971619) and the Overseas Expertise Introduction Project for Discipline Innovation (111 Project D18008) and by grants from Australia Research Council to the ARC Centre of Excellence in Plant Cell Walls (CE1101007) to A.B., M.S.D., K.L.J. and W.Z. M.H.C. acknowledges funding from the Novo Nordisk Foundation (grant nos. NNF18OC0053048 and NNF20OC0065094) and the Carlsberg Foundation (grant no. CF19-0072).