Valproic acid influences the expression of genes implicated with hyperglycaemia-induced complement and coagulation pathways.
Marina Barreto Felisbino, Mark Ziemann, Ishant Khurana, Jun Okabe, Keith Al-Hasani, Scott Maxwell, KN Harikrishnan, Camila Borges Martins de Oliveira, Maria Luiza S Mello, Assam El-Osta
Scientific Reports | Published : 2021
Because the liver plays a major role in metabolic homeostasis and secretion of clotting factors and inflammatory innate immune proteins, there is interest in understanding the mechanisms of hepatic cell activation under hyperglycaemia and whether this can be attenuated pharmacologically. We have previously shown that hyperglycaemia stimulates major changes in chromatin organization and metabolism in hepatocytes, and that the histone deacetylase inhibitor valproic acid (VPA) is able to reverse some of these metabolic changes. In this study, we have used RNA-sequencing (RNA-seq) to investigate how VPA influences gene expression in hepatocytes. Interesting, we observed that VPA attenuates hyper..View full abstract