White pupae phenotype of tephritids is caused by parallel mutations of a MFS transporter
Christopher M Ward, Roswitha A Aumann, Mark A Whitehead, Katerina Nikolouli, Gary Leveque, Georgia Gouvi, Elisabeth Fung, Sarah J Reiling, Haig Djambazian, Margaret A Hughes, Sam Whiteford, Carlos Caceres-Barrios, Thu NM Nguyen, Amanda Choo, Peter Crisp, Sheina B Sim, Scott M Geib, Frantisek Marec, Irina Haecker, Jiannis Ragoussis Show all
Nature Communications | NATURE RESEARCH | Published : 2021
Mass releases of sterilized male insects, in the frame of sterile insect technique programs, have helped suppress insect pest populations since the 1950s. In the major horticultural pests Bactrocera dorsalis, Ceratitis capitata, and Zeugodacus cucurbitae, a key phenotype white pupae (wp) has been used for decades to selectively remove females before releases, yet the gene responsible remained unknown. Here, we use classical and modern genetic approaches to identify and functionally characterize causal wp- mutations in these distantly related fruit fly species. We find that the wp phenotype is produced by parallel mutations in a single, conserved gene. CRISPR/Cas9-mediated knockout of the wp ..View full abstract
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