Journal article

Examination of Novel Immunomodulatory Effects of L-Sulforaphane

Nadia Mazarakis, Jeremy Anderson, Zheng Quan Toh, Rachel A Higgins, Lien Anh Ha Do, Rodney B Luwor, Kenneth J Snibson, Tom C Karagiannis, Paul Licciardi

NUTRIENTS | MDPI | Published : 2021

Abstract

The dietary isothiocyanate L-sulforaphane (LSF), derived from cruciferous vegetables, is reported to have several beneficial biological properties, including anti-inflammatory and immunomodulatory effects. However, there is limited data on how LSF modulates these effects in human immune cells. The present study was designed to investigate the immunomodulatory effects of LSF (10 µM and 50 µM) on peripheral blood mononuclear cell (PBMC) populations and cytokine secretion in healthy adult volunteers (n = 14), in the presence or absence of bacterial (lipopolysaccharide) and viral (imiquimod) toll-like receptor (TLRs) stimulations. Here, we found that LSF reduced pro-inflammatory cytokines interl..

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Funding Acknowledgements

The authors would like to acknowledge the use of the facilities provided by Flow Cytometry and Imaging at Murdoch Children's Research Institute (MCRI) and particularly, the expert assistance from Drs Matt Burton and Eleanor Jones. We would also like to thank the internship students Gloria Chew, Rena Tan Yan Ping, and Yi Ting Ma for their contribution towards this project. PVL is an NHMRC CDF2 recipient. TCK is supported by an Australian Research Council Future Fellowship and the Epigenomic Medicine Laboratory is supported by McCord Research (Iowa, IA, United States). NM and JA are supported by Australian Government Research Training Program Scholarships. This work was also supported by the Victorian Government's Operational Infrastructure Support Program. Graphical abstract created with BioRender.com (accessed on 10 February 2021).