Journal article

Intracellular bacteria are common and taxonomically diverse in cultured and in hospite algal endosymbionts of coral reefs

Justin Maire, Sam K Girvan, Sophie E Barkla, Alexis Perez-Gonzalez, David J Suggett, Linda L Blackall, Madeleine JH van Oppen

ISME JOURNAL | SPRINGERNATURE | Published : 2021

Abstract

Corals house a variety of microorganisms which they depend on for their survival, including endosymbiotic dinoflagellates (Symbiodiniaceae) and bacteria. While cnidarian-microorganism interactions are widely studied, Symbiodiniaceae-bacteria interactions are only just beginning to receive attention. Here, we describe the localization and composition of the bacterial communities associated with cultures of 11 Symbiodiniaceae strains from nine species and six genera. Three-dimensional confocal laser scanning and electron microscopy revealed bacteria are present inside the Symbiodiniaceae cells as well as closely associated with their external cell surface. Bacterial pure cultures and 16S rRNA ..

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Grants

Awarded by Australian Research Council Laureate Fellowship


Awarded by Australian Research Council


Funding Acknowledgements

This research was supported by the Australian Research Council Laureate Fellowship FL180100036 (to MJHvO) and the Australian Research Council (grant ID: DP160101468) to MJHvO and LLB. We thank the Biosciences Microscopy Unit (University of Melbourne) and the Biological Optical Microscopy Platform for the use of their confocal and scanning electron microscopes, and particularly Dr Allison van de Meene and Dr Gabriela Segal for their valuable assistance. We are grateful to: Dr. Katarina Damjanovic and Dr. Leon M. Hartman for their valuable help in developing codes for metabarcoding data analysis; Giada Tortorelli for assistance in Symbiodiniaceae maintenance; Dr. Wing Chan and Talisa Doring for providing Galaxea fascicularis polyps; Dr. Cindy Castelle for her help in creating phylogenetic trees.