Journal article

Chromosomes distribute randomly to, but not within, human neutrophil nuclear lobes

Christine R Keenan, Michael J Mlodzianoski, Hannah D Coughlan, Naiara G Bediaga, Gaetano Naselli, Erin C Lucas, Qike Wang, Carolyn A de Graaf, Douglas J Hilton, Leonard C Harrison, Gordon K Smyth, Kelly L Rogers, Thomas Boudier, Rhys S Allan, Timothy M Johanson

ISCIENCE | CELL PRESS | Published : 2021

Abstract

The proximity pattern and radial distribution of chromosome territories within spherical nuclei are random and non-random, respectively. Whether this distribution pattern is conserved in the partitioned or lobed nuclei of polymorphonuclear cells is unclear. Here we use chromosome paint technology to examine the chromosome territories of all 46 chromosomes in hundreds of single human neutrophils - an abundant and famously polymorphonuclear immune cell. By comparing the distribution of chromosomes to randomly shuffled controls and validating with orthogonal chromosome conformation capture technology, we show for the first time that human chromosomes randomly distribute to neutrophil nuclear lo..

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Grants

Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

The authors thank S. Johanson for constructive discussions, and the volunteers at theWalter and Eliza Hall Volunteer Blood Donor Registry. This work was supported by grants and fellowships from the National Health and Medical Research Council of Australia (T. M.J. #1124081, R.S.A. and T.M.J. #1049307, #1100451, G.K.S. #1058892, R.S.A. and G.K. S. #1158531, C.R.K. #1125436, D.J.H #1113577, L.C.H. #1080887 and #1037321). H.D.C. was supported by an H.C. Marian and E.H. Flack Fellowship. This study was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC Independent Research Institute Infrastructure Support scheme. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.