Cardiomyocytes Birefingence Imaging

Abstract

Quantitative Phase Microscopy is a simple imaging methodology for resolving internal morphology of unstained living specimens. It computes the 2D phase images of a specimen from a combination of bright field images. However, when used in conjunction with polarised light can lead to the simultaneous mapping of the phase and the retardation of an optically anisotropic specimen. Experimentally the procedure is implemented without the need of expensive compensators used in traditional methods.