Journal article

Macrophages provide a transient muscle stem cell niche via NAMPT secretion

Dhanushika Ratnayake, Phong D Nguyen, Fernando J Rossello, Verena C Wimmer, Jean L Tan, Laura A Galvis, Ziad Julier, Alasdair J Wood, Thomas Boudier, Abdulsalam I Isiaku, Silke Berger, Viola Oorschot, Carmen Sonntag, Kelly L Rogers, Christophe Marcelle, Graham J Lieschke, Mikael M Martino, Jeroen Bakkers, Peter D Currie

NATURE | NATURE RESEARCH | Published : 2021

Abstract

Skeletal muscle regenerates through the activation of resident stem cells. Termed satellite cells, these normally quiescent cells are induced to proliferate by wound-derived signals1. Identifying the source and nature of these cues has been hampered by an inability to visualize the complex cell interactions that occur within the wound. Here we use muscle injury models in zebrafish to systematically capture the interactions between satellite cells and the innate immune system after injury, in real time, throughout the repair process. This analysis revealed that a specific subset of macrophages 'dwell' within the injury, establishing a transient but obligate niche for stem cell proliferation. ..

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Grants

Awarded by EMBO Long Term Fellowship


Awarded by HFSPO Fellowship


Awarded by NWO-ZonMW Veni grant


Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

We thank Monash Micro Imaging and Monash Ramaciotti Centre for Cryo-Electron Microscopy for microscopy support, Hubrecht Institute single-cell sequencing facility for sequencing service, Hubrecht Institute flow cytometry core facility and Monash FlowCore for sorting of cells using FACS and Monash Fishcore staff for technical assistance, A. Kawakami for the mmp9:eGFP and mmp9:eGFP-NTR BAC transgenic lines, F. J. Tulenko for advice on phylogenetic tree construction, H. Janovjak for advice on in vitro assays, A.-M. Tichy, E. Gerrard and A. Ruparelia for technical assistance and B. T. Kile, P. Gonczy and R. P. Currie for comments on the manuscript. P.D.N. is supported by an EMBO Long Term Fellowship (ALTF1129-2015), HFSPO Fellowship (LT001404/2017-L) and a NWO-ZonMW Veni grant (016.186.017-3). This work was supported by the National Health and Medical Research Council of Australia (APP1041885, APP1136567, APP1104190, APP1159278 to P.D.C.; APP1044754, APP1069284, APP1086020 to G.J.L.; and APP1140229, APP1176213 to M.M.M.). The Australian Regenerative Medicine Institute is supported by grants from the State Government of Victoria and the Australian Government.