Journal article

Diagnosis of soil-transmitted helminths using the Kato-Katz technique: What is the influence of stirring, storage time and storage temperature on stool sample egg counts?

Felix Bosch, Marta S Palmeirim, Said M Ali, Shaali M Ame, Jan Hattendorf, Jennifer Keiser

PLoS Negl Trop Dis | Published : 2021

Abstract

BACKGROUND: Soil-transmitted helminths infect about one fifth of the world's population and have a negative impact on health. The Kato-Katz technique is the recommended method to detect soil-transmitted helminth eggs in stool samples, particularly in programmatic settings. However, some questions in its procedure remain. Our study aimed to investigate the effect of storage time, storage temperature and stirring of stool samples on fecal egg counts (FECs). METHODOLOGY/PRINCIPAL FINDINGS: In the framework of a clinical trial on Pemba Island, United Republic of Tanzania, 488 stool samples were collected from schoolchildren. These samples were evaluated in three experiments. In the first experim..

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University of Melbourne Researchers