SUGAR-seq enables simultaneous detection of glycans, epitopes, and the transcriptome in single cells
Conor J Kearney, Stephin J Vervoort, Kelly M Ramsbottom, Izabela Todorovski, Emily J Lelliott, Magnus Zethoven, Lizzy Pijpers, Ben P Martin, Timothy Semple, Luciano Martelotto, Joseph A Trapani, Ian A Parish, Nichollas E Scott, Jane Oliaro, Ricky W Johnstone
SCIENCE ADVANCES | AMER ASSOC ADVANCEMENT SCIENCE | Published : 2021
Multimodal single-cell RNA sequencing enables the precise mapping of transcriptional and phenotypic features of cellular differentiation states but does not allow for simultaneous integration of critical posttranslational modification data. Here, we describe SUrface-protein Glycan And RNA-seq (SUGAR-seq), a method that enables detection and analysis of N-linked glycosylation, extracellular epitopes, and the transcriptome at the single-cell level. Integrated SUGAR-seq and glycoproteome analysis identified tumor-infiltrating T cells with unique surface glycan properties that report their epigenetic and functional state.
C.J.K. was supported by a National Health and Medical Research Council of Australia (NHMRC) early career fellowship. S.J.V. was supported by a Rubicon postdoctoral fellowship, the Netherlands and a Peter Mac foundation grant. Work from the Johnstone Lab (R.W.J.) was supported by the Cancer Council Victoria (CCV), NHMRC, and The Kids' Cancer Project. Work from the Oliaro laboratory (J.O.) was supported by NHMRC and the National Breast Cancer Foundation (NBCF).