Journal article
In vitro effects of Type i interferons (IFNτ and IFNα) on bovine hepatocytes cultured with or without Kupffer cells
KJ Endriß, MM Meyerholz, T Fischbach, L Brimmers, C Pfarrer, CD Marth, M Schmicke
Reproduction Fertility and Development | CSIRO PUBLISHING | Published : 2021
DOI: 10.1071/RD20278
Abstract
In cattle, maternal recognition of early pregnancy depends on the effects of the embryonic signal interferon (IFN)-τ. IFN-stimulated genes have been upregulated in the maternal liver during early pregnancy. In this study, primary hepatocyte cell culture models were evaluated for their suitability to test Type I IFN effects in vitro. The expression of target genes (interferon-stimulated gene 15 (ISG-15), interferon-induced GTP-binding protein (MX-1), C-X-C motif chemokine 10 (CXCL-10), CXCL-5, insulin-like growth factor 1 (IGF-1), IGF binding protein 2 (IGFBP-2)) was measured using reverse transcription-quantitative polymerase chain reaction in hepatocytes from monoculture or in indirect cocu..
View full abstractGrants
Funding Acknowledgements
The authors thank the J. and G. Bruns Foundation for financial support. The authors also thank Martina Baumgarten, Angela Jordan and Sandra Wilkening. Finally, the authors thank the staff and the leadership of the Clinic for Cattle, University of Veterinary Medicine Hanover for their support during sample collection, especially W. Kehler.