Altered Corneal Epithelial Dendritic Cell Morphology and Phenotype Following Acute Exposure to Hyperosmolar Saline
Kirthana Senthil, Haihan Jiao, Laura E Downie, Holly R Chinnery
Investigative Ophthalmology and Visual Science | Association for Research in Vision and Ophthalmology | Published : 2021
Purpose: The purpose of this study was to assess the morphological and phenotypic responses of corneal epithelial dendritic cells (DCs) to acute topical hyperosmolar stress, given a pathogenic role for tear hyperosmolarity in dry eye disease (DED). Methods: C57BL/6J mice were anesthetized and received 350 mOsm/L (physiological; n = 5 mice), 450 mOsm/L (n = 6), or 600 mOsm/L (n = 6) saline on a randomly assigned eye. Corneas were harvested 2 hours later. Immunofluorescent staining was performed using CD45, CD86, and CD68 antibodies to investigate DC morphology (density, viability, field area, circularity, and dendritic complexity) and immunological phenotype. Flow cytometry was used to conf..View full abstract
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Awarded by National Health and Medical Research Council
Funding support (to H.R.C.) provided by the National Health and Medical Research Council (APP1126540).