Application of genomics to understand salt tolerance in lentil

Abstract

Soil salinity is a major abiotic stress, limiting lentil productivity worldwide. Understanding the genetic basis of salt tolerance is vital to develop tolerant varieties. A diversity panel consist-ing of 276 lentil accessions was screened in a previous study through traditional and image‐based approaches to quantify growth under salt stress. Genotyping was performed using two contrasting methods, targeted (tGBS) and transcriptome (GBS‐t) genotyping‐by‐sequencing, to evaluate the most appropriate methodology. tGBS revealed the highest number of single‐base variants (SNPs) (c. 56,349), and markers were more evenly distributed across the genome compared to GBS‐t. A genome‐wide association study..