The gamma H2AX DSB marker may not be a suitable biodosimeter to measure the biological MRT valley dose
Jessica A Ventura, Jacqueline F Donoghue, Cameron J Nowell, Leonie M Cann, Liam RJ Day, Lloyd ML Smyth, Helen B Forrester, Peter AW Rogers, Jeffrey C Crosbie
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | TAYLOR & FRANCIS LTD | Published : 2021
PURPOSE: γH2AX biodosimetry has been proposed as an alternative dosimetry method for microbeam radiation therapy (MRT) because conventional dosimeters, such as ionization chambers, lack the spatial resolution required to accurately measure the MRT valley dose. Here we investigated whether γH2AX biodosimetry should be used to measure the biological valley dose of MRT-irradiated mammalian cells. MATERIALS AND METHODS: We irradiated human skin fibroblasts and mouse skin flaps with synchrotron MRT and broad beam (BB) radiation. BB doses of 1-5 Gy were used to generate a calibration curve in order to estimate the biological MRT valley dose using the γH2AX assay. RESULTS: Our key finding was that ..View full abstract
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Awarded by Australian National Health and Medical Research Council (NHMRC)
This study was supported by the Australian National Health and Medical Research Council (NHMRC) grant no. APP1061772. J.A.Ventura was supported by the Felix Meyer Scholarship in Obstetrics and Gynaecology at the Faculty of Medicine, Dentistry and Health Sciences, the University of Melbourne.