Journal article

Retinal ganglion cell dysfunction in mice following acute intraocular pressure is exacerbated by P2X7 receptor knockout

Anna YM Wang, Vickie HY Wong, Pei Ying Lee, Bang V Bui, Stefanie Dudczig, Kirstan A Vessey, Erica L Fletcher

SCIENTIFIC REPORTS | NATURE RESEARCH | Published : 2021

Abstract

There is increasing evidence for the vulnerability of specific retinal ganglion cell (RGC) types in those with glaucoma and in animal models. In addition, the P2X7-receptor (P2X7-R) has been suggested to contribute to RGC death following stimulation and elevated IOP, though its role in RGC dysfunction prior to death has not been examined. Therefore, we examined the effect of an acute, non-ischemic intraocular pressure (IOP) insult (50 mmHg for 30 min) on RGC function in wildtype mice and P2X7-R knockout (P2X7-KO) mice. We examined retinal function using electroretinogram recordings and individual RGC responses using multielectrode arrays, 3 days following acute IOP elevation. Immunohistochem..

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Grants

Awarded by National Health & Medical Research Council of Australia


Awarded by Australian Research Council


Funding Acknowledgements

This work was funded by the National Health & Medical Research Council of Australia project Grant APP1138253 (ELF/KAV) and APP1138509 (ELF). BVB is supported by an Australian Research Council future fellowship (130100338). All experiments and handling of animals were conducted in compliance with the standards of the Association of Vision Research and Ophthalmology (ARVO) Statement for the Use of Animals in Ophthalmic and Vision Research as well as the institutional guidelines of The University of Melbourne Animal Ethics Committee (AEC) (Ethics ID 1614030).