Molecular diagnosis of scabies using a novel probe-based polymerase chain reaction assay targeting high-copy number repetitive sequences in the Sarcoptes scabiei genome
Lena Chng, Deborah C Holt, Matt Field, Joshua R Francis, Dev Tilakaratne, Milou H Dekkers, Greg Robinson, Kate Mounsey, Rebecca Pavlos, Asha C Bowen, Katja Fischer, Anthony T Papenfuss, Robin B Gasser, Pasi K Korhonen, Bart J Currie, James S McCarthy, Cielo Pasay
PLOS NEGLECTED TROPICAL DISEASES | PUBLIC LIBRARY SCIENCE | Published : 2021
BACKGROUND: The suboptimal sensitivity and specificity of available diagnostic methods for scabies hampers clinical management, trials of new therapies and epidemiologic studies. Additionally, parasitologic diagnosis by microscopic examination of skin scrapings requires sample collection with a sharp scalpel blade, causing discomfort to patients and difficulty in children. Polymerase chain reaction (PCR)-based diagnostic assays, combined with non-invasive sampling methods, represent an attractive approach. In this study, we aimed to develop a real-time probe-based PCR test for scabies, test a non-invasive sampling method and evaluate its diagnostic performance in two clinical settings. METHO..View full abstract
Awarded by National Health and Medical Research Council Grant (Improving Health Outcomes in the Tropical North: A Multidisciplinary Collaboration [HOT NORTH])
This study was funded by a National Health and Medical Research Council Grant (Improving Health Outcomes in the Tropical North: A Multidisciplinary Collaboration [HOT NORTH], GNT1131932) [BJC, JMC, ACB]. No author received a salary from this Grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.