Journal article

Single-cell RNA sequencing reveals developmental heterogeneity among Plasmodium berghei sporozoites

Anthony A Ruberto, Caitlin Bourke, Nicolas Merienne, Thomas Obadia, Rogerio Amino, Ivo Mueller

Scientific Reports | NATURE RESEARCH | Published : 2021

Abstract

In the malaria-causing parasite's life cycle, Plasmodium sporozoites must travel from the midgut of a mosquito to the salivary glands before they can infect a mammalian host. However, only a fraction of sporozoites complete the journey. Since salivary gland invasion is required for transmission of sporozoites, insights at the molecular level can contribute to strategies for malaria prevention. Recent advances in single-cell RNA sequencing provide an opportunity to assess sporozoite heterogeneity at a resolution unattainable by bulk RNA sequencing methods. In this study, we use a droplet-based single-cell RNA sequencing workflow to analyze the transcriptomes of over 8000 Plasmodium berghei sp..

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University of Melbourne Researchers

Grants

Awarded by Agence Nationale de la Recherche


Awarded by National Health and Medical Research Council of Australia (NHMRC)


Awarded by NHMRC Principal Research Fellowship


Funding Acknowledgements

We thank the team of the CEPIA at the Institut Pasteur for providing mosquitoes and P. berghei ANKA sporozoites, and Soffia Gunnarsdottir for proofreading the manuscript. This work was supported by the Agence Nationale de la Recherche (ANR-17-CE13-0025 to A.A.R); the National Health and Medical Research Council of Australia (NHMRC; 1092789 and 1134989 to I.M.); and a NHMRC Principal Research Fellowship (1155075 to I.M).