Journal article
The use of dried blood spots for the serological evaluation of SARS-CoV-2 antibodies
ZQ Toh, RA Higgins, J Anderson, N Mazarakis, LAH Do, K Rautenbacher, P Ramos, K Dohle, S Tosif, N Crawford, K Mulholland, PV Licciardi
Journal of Public Health United Kingdom | Published : 2022
Abstract
Background: To determine if dried blood spot specimens (DBS) can reliably detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies, we compared the SARS-CoV-2 IgG antibody response in paired serum and eluates from DBS specimens. Methods: A total of 95 paired DBS and serum samples were collected from 74 participants (aged 1-63 years) as part of a household cohort study in Melbourne, Australia. SARS-CoV-2 IgG antibodies specific for the receptor-binding domain (RBD) and S1 proteins between serum and eluates from DBS specimens were compared using an FDA-approved ELISA method. Results: Among the 74 participants, 42% (31/74) were children and the rest were adults. A total of..
View full abstractGrants
Funding Acknowledgements
This work was supported by the Royal Children's Hospital Foundation and the MCRI Infection and Immunity Theme, as well as the Victorian Government's Medical Research Operational Infrastructure Support Program. P.V.L. is supported by NHMRC Career Development Fellowship.