Pre-mitotic genome re-organisation bookends the B cell differentiation process
Wing Fuk Chan, Hannah D Coughlan, Jie HS Zhou, Christine R Keenan, Naiara G Bediaga, Philip D Hodgkin, Gordon K Smyth, Timothy M Johanson, Rhys S Allan
Nature Communications | NATURE RESEARCH | Published : 2021
During cellular differentiation chromosome conformation is intricately remodelled to support the lineage-specific transcriptional programs required for initiating and maintaining lineage identity. When these changes occur in relation to cell cycle, division and time in response to cellular activation and differentiation signals has yet to be explored, although it has been proposed to occur during DNA synthesis or after mitosis. Here, we elucidate the chromosome conformational changes in B lymphocytes as they differentiate and expand from a naive, quiescent state into antibody secreting plasma cells. We find gene-regulatory chromosome reorganization in late G1 phase before the first division,..View full abstract
Awarded by National Health and Medical Research Council of Australia
Awarded by Australian Research Council
We thank the staff of the core facilities at the Walter and Eliza Hall Institute. This work was supported by grants and fellowships from the H.C. Marian and E.H. Flack Fellowship (H.D.C.), the National Health and Medical Research Council of Australia (C.R.K #1125436, P.D.H. #1054925, T.M.J #1124081, R.S.A #1100451, G.K.S & R.S.A #1158531), and the Australian Research Council (R.S.A. #130100541). This study was made possible through Victorian State Government Operational Infrastructure Support, the Australian Government NHMRC Independent Research Institute Infrastructure Support scheme, and the Australian Cancer Research Fund.