ENU Mutagenesis Screen Identifies a Novel Regulator of Platelet Production.
William S Stevenson, Donald Metcalf, Douglas J Hilton, Andrew W Roberts, Warren S Alexander
Blood | American Society of Hematology | Published : 2004
Abstract The genes that regulate variation in steady-state platelet count are largely unknown. We have attempted to identify novel genes that regulate platelet production by creating heritable models of thrombocytosis in mice by randomly inducing point mutations in the genome with chemical mutagenesis. Mice with a high platelet count were identified in the third generation of an ENU mutagenesis screen and several different lines of thrombocytotic mice have been established. Subsequent breeding of one line has demonstrated that this mutation, called Plt2, is inherited in an autosomal recessive manner. Automated platelet counts for Plt2 homozygote mice were 1997±6..View full abstract