Journal article

Detection of SARS- CoV-2 in saliva: implications for specimen transport and storage

Eloise Williams, Nicole Isles, Brian Chong, Katherine Bond, Yano Yoga, Julian Druce, Mike Catton, Susan A Ballard, Benjamin P Howden, Deborah A Williamson

JOURNAL OF MEDICAL MICROBIOLOGY | MICROBIOLOGY SOC | Published : 2021

Abstract

Saliva has recently been proposed as a suitable specimen for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Use of saliva as a diagnostic specimen may present opportunities for SARS-CoV-2 reverse transcription polymerase chain reaction (RT-PCR) testing in remote and low-resource settings. Determining the stability of SARS-CoV-2 RNA in saliva over time is an important step in determining optimal storage and transport times. We undertook an in vitro study to assess whether SARS-CoV-2 could be detected in contrived saliva samples. The contrived saliva samples comprised 10 ml pooled saliva spiked with gamma-irradiated SARS-CoV-2 to achieve a concentration of 2.58×..

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