PF388 HAT-PCR IS MORE SENSITIVE THAN FLOW FOR QUANTIFYING MRD IN CHRONIC LYMPHOCYTIC LEUKEMIA

Abstract

Sensitive quantification of minimal residual disease (MRD) is becoming important in chronic lymphocytic leukaemia (CLL) owing to the development of highly effective forms of treatment. Flow cytometry is widely used for quantification of MRD. HAT‐PCR (High Annealing Temperature or High A/T)‐PCR is a recently developed method which markedly decreases nonspecificity and thus enables measurement of MRD usually down to 5 x 10–7. Its simplicity and low cost make it particularly valuable for repeated measurement of MRD. To compare flow cytometry and HAT‐PCR for measurement of MRD in CLL. We compared measurement of MRD by HAT‐PCR with measurement of MRD by flow cytometry using samples obtained at va..