Journal article

IMPLICON: an ultra-deep sequencing method to uncover DNA methylation at imprinted regions

Tajda Klobucar, Elisa Kreibich, Felix Krueger, Maria Arez, Duarte Polvora-Brandao, Ferdinand von Meyenn, Simao Teixeira da Rocha, Melanie Eckersley-Maslin

NUCLEIC ACIDS RESEARCH | OXFORD UNIV PRESS | Published : 2020

Abstract

Genomic imprinting is an epigenetic phenomenon leading to parental allele-specific expression. Dosage of imprinted genes is crucial for normal development and its dysregulation accounts for several human disorders. This unusual expression pattern is mostly dictated by differences in DNA methylation between parental alleles at specific regulatory elements known as imprinting control regions (ICRs). Although several approaches can be used for methylation inspection, we lack an easy and cost-effective method to simultaneously measure DNA methylation at multiple imprinted regions. Here, we present IMPLICON, a high-throughput method measuring DNA methylation levels at imprinted regions with base-..

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Grants

Awarded by BBSRC Discovery Fellowship


Awarded by EMBO Fellowship


Awarded by Fundacao para a Ciencia e Tecnologia (FCT) Ministerio da Ciencia, Tecnologia e Ensino Superior (MCTES), Portugal


Awarded by FCT/MCTES


Funding Acknowledgements

Babraham Institute Translational Advisory Group award (to M.E.-M. and F.v.M.); M.E.-M. is supported by a BBSRC Discovery Fellowship [BB/T009713/1]; EMBO Fellowship [ALTF938-2014]; Marie Sklodowska-Curie Individual Fellowship; Work in S.T.d.R.'s team at iMM JLA was supported by Fundacao para a Ciencia e Tecnologia (FCT) Ministerio da Ciencia, Tecnologia e Ensino Superior (MCTES), Portugal [PTDC/BEX-BCM/2612/2014, PTDC/BIA-MOL/29320/2017 IC & DT]; S.T.d.R. has a CEECUIND/01234/207 assistant research contract from FCT/MCTES; T.K.'s work was supported by Erasmus+ and University Foundation of eng. Lenar.ci.c Milan at the University of Ljubljana. Funding for open access charge: accounts payable, Babraham Institute.