Journal article
IMPLICON: An ultra-deep sequencing method to uncover DNA methylation at imprinted regions
T Klobučar, E Kreibich, F Krueger, M Arez, D Pólvora-Brandão, F Von Meyenn, ST Da Rocha, M Eckersley-Maslin
Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2020
DOI: 10.1093/nar/gkaa567
Open access
Abstract
Genomic imprinting is an epigenetic phenomenon leading to parental allele-specific expression. Dosage of imprinted genes is crucial for normal development and its dysregulation accounts for several human disorders. This unusual expression pattern is mostly dictated by differences in DNA methylation between parental alleles at specific regulatory elements known as imprinting control regions (ICRs). Although several approaches can be used for methylation inspection, we lack an easy and cost-effective method to simultaneously measure DNA methylation at multiple imprinted regions. Here, we present IMPLICON, a high-throughput method measuring DNA methylation levels at imprinted regions with base-..
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Funding Acknowledgements
Babraham Institute Translational Advisory Group award (to M.E.-M. and F.v.M.); M.E.-M. is supported by a BBSRC Discovery Fellowship [BB/T009713/1]; EMBO Fellowship [ALTF938-2014]; Marie Sklodowska-Curie Individual Fellowship; Work in S.T.d.R.'s team at iMM JLA was supported by Fundacao para a Ciencia e Tecnologia (FCT) Ministerio da Ciencia, Tecnologia e Ensino Superior (MCTES), Portugal [PTDC/BEX-BCM/2612/2014, PTDC/BIA-MOL/29320/2017 IC & DT]; S.T.d.R. has a CEECUIND/01234/207 assistant research contract from FCT/MCTES; T.K.'s work was supported by Erasmus+ and University Foundation of eng. Lenar.ci.c Milan at the University of Ljubljana. Funding for open access charge: accounts payable, Babraham Institute.