Journal article
Establishment of porcine and human expanded potential stem cells
X Gao, M Nowak-Imialek, X Chen, D Chen, D Herrmann, D Ruan, ACH Chen, MA Eckersley-Maslin, S Ahmad, YL Lee, T Kobayashi, D Ryan, J Zhong, J Zhu, J Wu, G Lan, S Petkov, J Yang, L Antunes, LS Campos Show all
Nature Cell Biology | NATURE PORTFOLIO | Published : 2019
Abstract
We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the critical molecular pathways that predispose their differentiation. EPSCs had enriched molecular signatures of blastomeres and possessed developmental potency for all embryonic and extra-embryonic cell lineages. Here, we report the derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro. Under similar conditions, human embryonic stem cells and induced pluripotent stem cells can be converted, or somatic cells di..
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Awarded by Medizinischen Hochschule Hannover
Funding Acknowledgements
We thank our colleagues of the Wellcome Trust Sanger Institute core facilities for their generous support (J. Bussell, Y. Hooks, N. Smerdon, B. L. Ng and J. Graham) and A. Moffett for critical comments. We thank B. Petersen for performing the surgical embryo transfers and the staff of the experimental pig facility at the Friedrich-Loeffler-Institut Mariensee for their competent and enduring assistance. We acknowledge the following funding and support: the Wellcome Trust (grant nos. 098051 and 206194) to the Sanger Institute and the University of Hong Kong internal funding (P. Liu); a Wellcome Trust Clinical PhD Fellowship for Academic Clinicians (D.J.R.); a PhD fellowship from the Portuguese Foundation for Science and Technology, FCT (grant no. SFRH/BD/84964/2012; L.A.); a Marie Sklodowska-Curie Individual Fellowship (M.A.E.-M.); the BBSRC (grant no. BB/K010867/1), Wellcome Trust (grant no. 095645/Z/11/Z), EU EpiGeneSys and BLUEPRINT (W.R.); a Chongqing Agriculture Development Grant (grant no. 17407 to L.P.G.,Z.H.L. and Y.H.); REBIRTH project no. 9.1, Hannover Medical School (H.N.); Shuguang Planning of Shanghai Municipal Education Commission (grant no. 16SG14) and the National Key Research and Development Program (grant no. 2017YFA0104500; L. Lu); the China Postdoctoral Science Foundation (grant no. 2017M622795; D.C.); the Strategic Priority Research Program of CAS (grant nos. XDA16030503 and XDA16030501), the National Key Research and Development Program of China Stem Cell and Translational Research (grant no. 2017YFA0105103) and Key Research and Development Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory (grant no. 2018GZR110104004; L.Lai); the Shenzhen Municipal Government of China (DRC-SZ [2016] 884; Z.S.);the NHMRC of Australia (Senior Principal Research Fellowship grant no. 1110751; P.P.L.T.); the GRF of Hong Kong (grant nos 17119117 and 17107915) and the National Natural Science Foundation (grant nos. 81671579 (L. Lu), 31471398 (W.S.B.Y.)and U1804281 (Y. Zhang)).