Journal article

Dppa2 and Dppa4 directly regulate the Dux-driven zygotic transcriptional program

Melanie Eckersley-Maslin, Celia Alda-Catalinas, Marloes Blotenburg, Elisa Kreibich, Christel Krueger, Wolf Reik

Genes & Development | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | Published : 2019

Abstract

The molecular regulation of zygotic genome activation (ZGA) in mammals remains an exciting area of research. Primed mouse embryonic stem cells contain a rare subset of "2C-like" cells that are epigenetically and transcriptionally similar to the two-cell embryo and thus represent an in vitro approximation for studying ZGA transcription regulation. Recently, the transcription factor Dux, expressed in the minor wave of ZGA, was described to activate many downstream ZGA transcripts. However, it remains unknown what upstream maternal factors initiate ZGA in either a Dux-dependent or Dux-independent manner. Here we performed a candidate-based overexpression screen, identifying, among others, devel..

View full abstract

Grants

Awarded by EMBO Fellowship


Awarded by Biotechnology and Biological Sciences Research Council


Awarded by Wellcome Trust


Funding Acknowledgements

We thank all members of the Reik laboratory for helpful discussions, and Aled Parry for reading the manuscript. We also thank Felix Krueger for processing sequencing data and assistance with repeat mapping, Simon Andrews for bioinformatics support, Nathalie Smerdon for assistance with high-throughput sequencing, and Rachael Walker for assistance with flow cytometry. We also thank Didier Trono (Ecole Polytechnique Federale de Lausanne) for kindly providing Dux knockout ESCs and Dux constructs. M.E.-M. was supported by an EMBO Fellowship (ALTF938-2014) and a Marie Sklodowska-Curie Individual Fellowship. M.B. and E.K. were supported by Erasmus Grants. Research in the Reik laboratory is supported by the Biotechnology and Biological Sciences Research Council (BB/K010867/1), Wellcome Trust (095645/Z/11/Z), and European Union Epigenesys.