Journal article

Establishment of mouse expanded potential stem cells

Jian Yang, David J Ryan, Wei Wang, Jason Cheuk-Ho Tsang, Guocheng Lan, Hideki Masaki, Xuefei Gao, Liliana Antunes, Yong Yu, Zhexin Zhu, Juexuan Wang, Aleksandra A Kolodziejczyk, Lia S Campos, Cui Wang, Fengtang Yang, Zhen Zhong, Beiyuan Fu, Melanie A Eckersley-Maslin, Michael Woods, Yosuke Tanaka Show all

Nature | NATURE PUBLISHING GROUP | Published : 2017


Mouse embryonic stem cells derived from the epiblast contribute to the somatic lineages and the germline but are excluded from the extra-embryonic tissues that are derived from the trophectoderm and the primitive endoderm upon reintroduction to the blastocyst. Here we report that cultures of expanded potential stem cells can be established from individual eight-cell blastomeres, and by direct conversion of mouse embryonic stem cells and induced pluripotent stem cells. Remarkably, a single expanded potential stem cell can contribute both to the embryo proper and to the trophectoderm lineages in a chimaera assay. Bona fide trophoblast stem cell lines and extra-embryonic endoderm stem cells can..

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Awarded by Portuguese Foundation for Science and Technology, FCT

Awarded by National Institutes of Health

Awarded by European Molecular Biology Organization

Awarded by Biotechnology and Biological Sciences Research Council

Awarded by Wellcome Trust

Awarded by Bloodwise

Awarded by Cancer Research UK

Awarded by National Health and Medical Research Council

Awarded by National Natural Science Foundation of China

Awarded by National Key Research and Development Program

Awarded by Medical Research Council

Funding Acknowledgements

We thank colleagues of the Research Support Facility (B. Doe, S. Newman, E. Grau and others), Y. Hooks, Sequencing (N. Smerdon) and FACS core facilities (B. L. Ng and J. Graham) at the Sanger Institute, the animal facility at the Cancer Research UK Cambridge Institute, and P. Humphreys of the University of Cambridge, for technical support; S. Gerety for the fluorescence stereo microscope, J. K. Kim for informatics advice, S. Rice for help on DNA bisulfite sequencing analysis; J. Nichols, A. Martinez Arias, K. McDole and Y. Zheng for reagents; J. Thomson, E. Robertson and A. Ang for comments. We acknowledge the following funding and support: Wellcome Trust Clinical PhD Fellowship for Academic Clinicians (D.J.R.); PhD fellowship (Portuguese Foundation for Science and Technology, FCT (SFRH/BD/84964/2012)) (L.A.); Japan Society for the Promotion of Science fellowship (Y.T.); National Institutes of Health (RP-PG-0310-10002) (A.C.W.); European Molecular Biology Organization (ALTF938-2014) and Marie Sklodowska-Curie Individual Fellowship (M.A.E.-M.); Biotechnology and Biological Sciences Research Council (BB/K010867/1) and Wellcome Trust (095645/Z/11/Z) (W.R.); Bloodwise (12029), Cancer Research UK (C1163/A12765 and C1163/A21762) and Wellcome Trust core funding (SCI 097922/Z/11/Z) (B.G.); Leading Advanced Projects for Medical Innovation, Japan Agency for Medical Research and Development (H.N. and H.M.); National Health and Medical Research Council Senior Principal Research Fellowship (1110751) (P.P.L.T.); National Natural Science Foundation of China (81671579, 31370904, 30972691) and The National Key Research and Development Program (2017YFA0104500) (L.L.). P.L. thanks M. Stratton, A. Bradley, N. Copeland, N. Jenkins and J. Lupski for their encouragement in these experiments. P.L. is an affiliate faculty member of the Wellcome Trust-MRC Stem Cell Institute, University of Cambridge. The P.L. laboratory is supported by the Wellcome Trust (grant numbers 098051 and 206194).