Journal article

Investigating virus-host cell interactions: Comparative binding forces between hepatitis C virus-like particles and host cell receptors in 2D and 3D cell culture models

Simon Collett, Joseph Torresi, Linda Earnest Silveira, Vi Khanh Truong, Dale Christiansen, Bang M Tran, Elizabeth Vincan, Paul A Ramsland, Aaron Elbourne

JOURNAL OF COLLOID AND INTERFACE SCIENCE | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2021

Abstract

Cell cultures have been successfully used to study hepatitis C virus (HCV) for many years. However, most work has been done using traditional, 2-dimensional (2D) cell cultures (cells grown as a monolayer in growth flasks or dishes). Studies have shown that when cells are grown suspended in an extra-cellular-matrix-like material, they develop into spherical, 'organoid' arrangements of cells (3D growth) that display distinct differences in morphological and functional characteristics compared to 2D cell cultures. In liver organoids, one key difference is the development of clearly differentiated apical and basolateral surfaces separated and maintained by cellular tight junctions. This phenomen..

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Grants

Awarded by Jack Brockhoff Foundation (JBF)


Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

This research was performed in part at the RMIT Micro Nano Research Facility (MNRF) in the Victorian Node of the Australian National Fabrication Facility (ANFF). We acknowledge the facilities, and the scientific and technical assistance of the RMIT Microscopy & Microanalysis Facility (RMMF), a linked laboratory of Microscopy Australia. S.C. is supported by a research training program stipend scholarship from the Australian Government, Department of Education and Training. A.E. acknowledges support from the Jack Brockhoff Foundation (JBF Grant number 4655-2019). BMT, JT and EV acknowledge support from the National Health and Medical Research Council of Australia (APP1181580).