Journal article

A conserved Zn2Cys6 transcription factor, identified in a spontaneous mutant from in vitro passaging, is involved in pathogenicity of the blackleg fungus Leptosphaeria maculans

Kylie R Chambers, Angela P Van de Wouw, Donald M Gardiner, Candace E Elliott, Alexander Idnurm



Continuous passaging in vitro can lead to the accumulation of changes in DNA sequence that potentially affect the properties of microbes, making them different from the original isolates. The identification of such genetic alterations is rare in fungi. A set of insertional mutants in the plant pathogenic fungus Leptosphaeria maculans, all derived from the same transformation experiment, had independent Agrobacterium T-DNA insertions and reduced pathogenicity on canola (Brassica napus). None of the insertions co-segregated in progeny from crosses with the reduction in pathogenicity. Genome sequences of three strains were analysed, and a mutation identified in a gene (ptf1, for pathogenicity-a..

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Awarded by Australian Grains Research and Development Corporation (GRDC)

Awarded by Australian Research Council

Funding Acknowledgements

This work was supported by the Australian Grains Research and Development Corporation (GRDC projects UM00050 and UM00051) and the Australian Research Council (project FT130100146) . We thank the University of Melbourne's Botany Foundation for support through the Gretna Weste scholarship and from the Robert Lipp Plant Science Memorial Research Fellowship.