OP019 In faecal microbiota transplantation (FMT) for ulcerative colitis, fusobacterium is associated with lack of remission, while metabolic shifts to starch degradation and short-chain fatty acid production are associated with remission (FOCUS study)

Abstract

In the FOCUS study, multidonor FMT was effective in the treatment of active ulcerative colitis (UC).1 Here we characterise the bacterial taxonomic and functional changes associated with outcome. A total of 314 fecal and 160 colonic biopsy samples were collected at specific intervals from 70 patients. A total of 113 fecal samples were collected from the 14 individual donors and 21 multidonor batches. DNA and RNA were extracted, RNA converted to cDNA, then 16S rRNA gene sequencing performed using 2x300 bp Illumina MiSeq chemistry. Sequences were analysed using MOTHUR. Shotgun metagenomics was performed on 285 fecal samples using 2x250bp HiSeq 2500 chemistry, with samples analysed using MetaPhl..