Characterization of the circulating and tissue-specific alterations to the lipidome in response to moderate and major cold stress in mice
Gerard Pernes, Pooranee K Morgan, Kevin Huynh, Natalie A Mellett, Peter J Meikle, Andrew J Murphy, Darren C Henstridge, Graeme I Lancaster
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2021
This study analyzed the effects of 24 h of cold stress (22°C or 5°C vs. mice maintained at 30 °C) on the plasma, brown adipose tissue (BAT), subcutaneous (SubQ) and epididymal (Epi) white adipose tissue (WAT), liver, and skeletal muscle lipidome of mice. Using mass spectrometry-lipidomics, 624 lipid species were detected, of which 239 were significantly altered in plasma, 134 in BAT, and 51 in the liver. In plasma, acylcarnitines and free fatty acids were markedly increased at 5°C. Plasma triacylglycerols (TGs) were reduced at 22°C and 5°C. We also identified ether lipids as a novel, cold-induced lipid class. In BAT, TGs were the principal lipid class affected by cold stress, being significa..View full abstract
This study was funded in part by the Victorian Governments Operational Support Program and the Heine Foundation. G. Pernes was supported by a Research Training Program (RTP) Stipend and a Baker Heart and Diabetes Institute Bright Sparks award. P. K. Morgan was supported by a joint Baker Heart and Diabetes Institute Research Scholarship-La Trobe University Scholarship. A. J. Murphy was supported by a CSL Centenary Fellowship. D. C. Henstridge was supported by the Shine on Foundation.