Robust correlations across six SARS-CoV-2 serology assays detecting distinct antibody features

LC Rowntree; BY Chua; S Nicholson; M Koutsakos; L Hensen; C Douros; K Selva; FL Mordant; CY Wong; JR Habel; W Zhang; X Jia; L Allen; DL Doolan; DC Jackson; AK Wheatley; SJ Kent; F Amanat; F Krammer; K Subbarao; AC Cheng; AW Chung; M Catton; THO Nguyen; CE van de Sandt; K Kedzierska

Journal article

Robust correlations across six SARS-CoV-2 serology assays detecting distinct antibody features

LC Rowntree, BY Chua, S Nicholson, M Koutsakos, L Hensen, C Douros, K Selva, FL Mordant, CY Wong, JR Habel, W Zhang, X Jia, L Allen, DL Doolan, DC Jackson, AK Wheatley, SJ Kent, F Amanat, F Krammer, K Subbarao Show all

Clinical and Translational Immunology | WILEY | Published : 2021

DOI: 10.1002/cti2.1258

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Abstract

Objectives: As the world transitions into a new era of the COVID-19 pandemic in which vaccines become available, there is an increasing demand for rapid reliable serological testing to identify individuals with levels of immunity considered protective by infection or vaccination. Methods: We used 34 SARS-CoV-2 samples to perform a rapid surrogate virus neutralisation test (sVNT), applicable to many laboratories as it circumvents the need for biosafety level-3 containment. We correlated results from the sVNT with five additional commonly used SARS-CoV-2 serology techniques: the microneutralisation test (MNT), in-house ELISAs, commercial Euroimmun- and Wantai-based ELISAs (RBD, spike and nucle..

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University of Melbourne Researchers

Louise Rowntree Author

Brendon Chua Author

Marios Koutsakos Author

Kevin John Selva Author

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Awarded by Department of Health, Australian Government

Funding Acknowledgements

We thank all the participants involved in the study; Bernie McCudden and Jenny Mitchell for support with the cohort; and Jill Garlick, Janine Roney, Anne Paterson and the research nurses at the Alfred Hospital. This work was supported by the Australian National Health and Medical Research Council (NHMRC) Leadership Investigator Grant to KK (#1173871), NHMRC Program Grant to DLD (#1132975), Research Grants Council of the Hong Kong Special Administrative Region, China (#T11-712/19-Ncdf) to KK, the Jack Ma Foundation to KK, KS and AWC, the Medical Research Future Fund (#2005544) to KK, SJK, AKW and AWC, the a2 Milk Foundation to KS, MRFF Award (#1202445) to KK, NHMRC Program Grant (#1071916) to KK. KK was supported by NHMRC Senior Research Fellowship (1102792), DLD by a NHMRC Principal Research Fellowship (#1137285) and KS by an NHMRC Investigator grant (#1177174). THON is supported by NHMRC EL1 Fellowship (#1194036). LH is supported by the Melbourne International Research Scholarship (MIRS) and the Melbourne International Fee Remission Scholarship from The University of Melbourne. JRH and WZ are supported by the Melbourne Research Scholarship from The University of Melbourne. XJ is supported by China Scholarship CouncilUniversity of Melbourne joint Scholarship. CES has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie SklodowskaCurie grant agreement (#792532). CES and KS received funding from the Doherty Collaborative Seed Grant. KK and AWC were supported by the University of Melbourne Dame Kate Campbell Fellowship. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health.