Journal article

Optimal preparation of SARS-CoV-2 viral transport medium for culture

Julie McAuley, Claire Fraser, Elena Paraskeva, Elizabeth Trajcevska, Michelle Sait, Nancy Wang, Eric Bert, Damian Purcell, Richard Strugnell

VIROLOGY JOURNAL | BMC | Published : 2021

Abstract

INTRODUCTION: The sudden arrival of the COVID-19 pandemic placed significant stresses on supply chains including viral transport medium (VTM). The VTM that was urgently required needed to support viral replication, as well as other routine diagnostic approaches. We describe the preparation and validation testing of VTM for rapidly expanding diagnostic testing, where the capacity of the VTM to preserve viral integrity, for culture, isolation and full sequence analysis, was maintained. METHODS: VTM was prepared using different methods of sterilization then 'spiked' with virus. The VTM was investigated using viral culture in Vero cells, and for nucleic acid detection by quantitative PCR. RESULT..

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Grants

Awarded by NHMRC


Funding Acknowledgements

This work was funded by an NHMRC Program Grant ID1092262 supporting RAS and NW and an unrestricted grant from the A2 milk foundation supporting DJP.