Journal article

Sex-Specific Control of Human Heart Maturation by the Progesterone Receptor

Choon Boon Sim, Belinda Phipson, Mark Ziemann, Haloom Rafehi, Richard J Mills, Kevin I Watt, Kwaku D Abu-Bonsrah, Ravi KR Kalathur, Holly K Voges, Doan T Dinh, Menno ter Huurne, Celine J Vivien, Antony Kaspi, Harikrishnan Kaipananickal, Alejandro Hidalgo, Leanne MD Delbridge, Rebecca L Robker, Paul Gregorevic, Cristobal G dos Remedios, Sean Lal Show all



BACKGROUND: Despite in-depth knowledge of the molecular mechanisms controlling embryonic heart development, little is known about the signals governing postnatal maturation of the human heart. METHODS: Single-nucleus RNA sequencing of 54 140 nuclei from 9 human donors was used to profile transcriptional changes in diverse cardiac cell types during maturation from fetal stages to adulthood. Bulk RNA sequencing and the Assay for Transposase-Accessible Chromatin using sequencing were used to further validate transcriptional changes and to profile alterations in the chromatin accessibility landscape in purified cardiomyocyte nuclei from 21 human donors. Functional validation studies of sex stero..

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Funding Acknowledgements

The authors received grant and fellowship support from the National Health and Medical Research Council of Australia (E.R.P., J.E.H., A.O., A.E.-O., B.P., R.R.), the Australian Research Council (E.R.P., A.E.-O., L.M.D.), the Heart Foundation of Australia (E.R.P. and J.E.H.), the Stafford Fox Medical Research Foundation (E.R.P.), and the Royal Children's Hospital Foundation (E.R.P., I.E.K., D.A.E., L.M.D.). MCRI is supported by the Victorian Government's Operational Infrastructure Support Program.