Complementation in trans of Porphyromonas gingivalis Lipopolysaccharide Biosynthetic Mutants Demonstrates Lipopolysaccharide Exchange
Michelle D Glew, Dhana G Gorasia, Paul J McMillan, Catherine A Butler, Paul D Veith, Eric C Reynolds
JOURNAL OF BACTERIOLOGY | AMER SOC MICROBIOLOGY | Published : 2021
Porphyromonas gingivalis, a bacterial pathogen contributing to human periodontitis, exports and anchors cargo proteins to its surface, enabling the production of black pigmentation using a type IX secretion system (T9SS) and conjugation to anionic lipopolysaccharide (A-LPS). To determine whether T9SS components need to be assembled in situ for correct secretion and A-LPS modification of cargo proteins, combinations of nonpigmented mutants lacking A-LPS or a T9SS component were mixed to investigate in trans complementation. Reacquisition of pigmentation occurred only between an A-LPS mutant and a T9SS mutant, which coincided with A-LPS modification of cargo proteins detected by Western blotti..View full abstract
Related Projects (1)
Awarded by Australian Government Department of Industry, Innovation and Science
Awarded by National Health and Medical Research Council of Australia
This study was supported by the Australian Government Department of Industry, Innovation and Science (grant ID 20080108) and the National Health and Medical Research Council of Australia (grant ID 1123866).