Rapid and Detailed Characterization of Transgene Insertion Sites in Genetically Modified Plants via Nanopore Sequencing
Paula A Giraldo, Hiroshi Shinozuka, German C Spangenberg, Kevin F Smith, Noel O Cogan
FRONTIERS IN PLANT SCIENCE | FRONTIERS MEDIA SA | Published : 2021
Molecular characterization of genetically modified plants can provide crucial information for the development of detection and identification methods, to comply with traceability, and labeling requirements prior to commercialization. Detailed description of the genetic modification was previously a challenging step in the safety assessment, since it required the use of laborious and time-consuming techniques. In this study an accurate, simple, and fast method was developed for molecular characterization of genetically modified (GM) plants, following a user-friendly workflow for researchers with limited bioinformatic capabilities. Three GM events from a diverse array of crop species-perennial..View full abstract
This work was supported by funding from Agriculture Victoria Research and an Australian Government Research Training Program Scholarship for PG.