In Vivo Quantification of Nanoparticle Association with Immune Cell Subsets in Blood
Yih Rue Ong, Robert De Rose, Angus PR Johnston
ADVANCED HEALTHCARE MATERIALS | WILEY | Published : 2021
Nanoparticles offer great promise for more effective drug delivery. However, their particulate nature typically results in rapid systemic clearance by immune cells in blood. Currently, to understand these interactions, nanoparticle association is probed ex vivo with whole blood. While ex vivo assays give important information about the relative cell association, they do not consider changes in immune cell homeostasis or the complex mixing behavior that occurs in vivo. To address this, a nanoparticle in vivo immune-cell association assay is developed to study the in vivo association of unmodified and poly(ethylene glycol) modified liposomes with immune cells, and compared this to the ex vivo ..View full abstract
Awarded by National Health and Medical Research Council
This research was supported by a National Health and Medical Research Council Project Grant (1129672, A.P.R.J.) and Career Development Fellowship (1141551, A.P.R.J.) as well as the Aus-tralian Research Council through the Centre of Excellence in Convergent Bio-Nano Science and Technology (A.P.R.J.). A.P.R.J. is also supported through the Monash University Lar-kin's Fellowship Scheme.