Journal article

Molecular Basis of Sulfosugar Selectivity in Sulfoglycolysis

Mahima Sharma, Palika Abayakoon, Ruwan Epa, Yi Jin, James P Lingford, Tomohiro Shimada, Masahiro Nakano, Janice W-Y Mui, Akira Ishihama, Ethan D Goddard-Borger, Gideon J Davies, Spencer J Williams

ACS CENTRAL SCIENCE | AMER CHEMICAL SOC | Published : 2021

Abstract

The sulfosugar sulfoquinovose (SQ) is produced by essentially all photosynthetic organisms on Earth and is metabolized by bacteria through the process of sulfoglycolysis. The sulfoglycolytic Embden-Meyerhof-Parnas pathway metabolizes SQ to produce dihydroxyacetone phosphate and sulfolactaldehyde and is analogous to the classical Embden-Meyerhof-Parnas glycolysis pathway for the metabolism of glucose-6-phosphate, though the former only provides one C3 fragment to central metabolism, with excretion of the other C3 fragment as dihydroxypropanesulfonate. Here, we report a comprehensive structural and biochemical analysis of the three core steps of sulfoglycolysis catalyzed by SQ isomerase, sulfo..

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Grants

Awarded by Australian Research Council


Awarded by Leverhulme Trust


Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

This work was supported by the Australian Research Council (DP180101957, DP210100233), the Leverhulme Trust (RPG-2017-190), MEXT Cooperative Research Program of Network Joint Research Center for Materials and Devices (AI and TS), the National Health and Medical Research Council of Australia (GNT1139546 and GNT1139549), and the Royal Society for the Ken Murray Research Professorship to G. J.D. We acknowledge support from The Walter and Eliza Hall Institute of Medical Research; the Australian Cancer Research Fund; and a Victorian State Government Operational Infrastructure support grant. We acknowledge the staff of the Diamond Light Source (U.K.) for provision of IO3, IO4, and IO4-1 beamline facilities (proposal numbers mx-13587, mx-18598, and mx24948). We thank Noriyuki Kodera (Kanazawa Univ.) for fabricating the electron beam deposited tips of AFM, and Christopher Bengt, Joe Joiner, and Prof. David Vocadlo for constructive advice.