Global reprogramming of virulence and antibiotic resistance in Pseudomonas aeruginosa by a single nucleotide polymorphism in elongation factor, fusA1
Eve A Maunders, Rory C Triniman, Joshua Western, Taufiq Rahman, Martin Welch
JOURNAL OF BIOLOGICAL CHEMISTRY | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | Published : 2020
Clinical isolates of the opportunistic pathogen Pseudomonas aeruginosa from patients with cystic fibrosis (CF) frequently contain mutations in the gene encoding an elongation factor, FusA1. Recent work has shown that fusA1 mutants often display elevated aminoglycoside resistance due to increased expression of the efflux pump, MexXY. However, we wondered whether these mutants might also be affected in other virulence-associated phenotypes. Here, we isolated a spontaneous gentamicin-resistant fusA1 mutant (FusA1P443L) in which mexXY expression was increased. Proteomic and transcriptomic analyses revealed that the fusA1 mutant also exhibited discrete changes in the expression of key pathogenici..View full abstract
Awarded by Medical Research Council (MRC) DTP Programme Studentship
This work was supported by Medical Research Council (MRC) DTP Programme Studentship MR/K50127X/1 (to E. A. M.) and by two flexible supplement awards (also from the MRC) for training in proteomic and transcriptomic analysis. R. C. T. was supported by a Ph.D. studentship from Hughes Hall Cambridge. Elements of the biochemical and genetic characterization were supported by a grant from the Evelyn Trust.