Journal article

Canonical ammonia oxidizers, rather than comammox Nitrospira, dominated autotrophic nitrification during the mineralization of organic substances in two paddy soils

Haiyang Liu, Hangwei Hu, Xing Huang, Tida Ge, Yongfu Li, Zhenke Zhu, Xingmei Liu, Wenfeng Tan, Zhongjun Jia, Hongjie Di, Jianming Xu, Yong Li

SOIL BIOLOGY & BIOCHEMISTRY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2021

Abstract

Chemoautotrophic canonical ammonia-oxidizers and complete ammonia oxidizers (comammox) are responsible for ammonia oxidation, which is the rate-limiting step of nitrification in terrestrial ecosystems. However, the relative importance of autotrophic nitrification and the related active nitrifiers during mineralization of organic substances with different C/N ratios have not been fully elucidated to date. In this study, 15N tracing and 13CO2-DNA-based stable isotope probing (DNA-SIP) techniques were employed to investigate the responses of soil nitrification processes and the active nitrifying phylotypes in acidic and neutral paddy soils with amendment of glutamine (Gln) or rice straw with lo..

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University of Melbourne Researchers

Grants

Awarded by National Key R&D Program of China


Awarded by National Natural Science Foundation of China


Awarded by Fundamental Research Funds for the Central Universities


Funding Acknowledgements

This work was supported by National Key R&D Program of China (2016YFD0200302), National Natural Science Foundation of China (41671249, 42007033) and Fundamental Research Funds for the Central Universities (2019QNA6011). The authors sincerely appreciate two anonymous reviewers acknowledging their insightful and helpful suggestions for improving the manuscript.