Journal article

Long-term effects of young-adult methamphetamine on dorsal raphe serotonin systems in mice: Role of brain-derived neurotrophic factor

Mauricio Sepulveda, Elizabeth E Manning, Andrea Gogos, Matthew Hale, Maarten van den Buuse

BRAIN RESEARCH | ELSEVIER | Published : 2021

Abstract

To assess the long-term effects of chronic adolescent methamphetamine (METH) treatment on the serotonin system in the brain, we used serotonin-1A receptor (5-HT1A) and serotonin transporter (SERT) autoradiography, and quantitative tryptophan-hydroxylase 2 (TPH2) immunohistochemistry in the raphe nuclei of mice. Because of the modulatory role of brain-derived neurotrophic factor (BDNF) on the serotonin system and the effects of METH, we included both BDNF heterozygous (HET) mice and wildtype (WT) controls. Male and female mice of both genotypes were treated with an escalating METH dose regimen from the age of 6-9 weeks. At least two weeks later, acute locomotor hyperactivity induced by a 5 mg..

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Grants

Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

This research was part-funded by the National Health and Medical Research Council of Australia (AG CDF 1108098, MvdB SRF 1041895). MS and EM were supported by Australian Postgraduate Awards. The Florey Institute of Neuroscience and Mental Health acknowledges the funding from the Victorian Government's Operational Infrastructure Support. These funding sources had no role in the study design, the collection, analysis and interpretation of the data, the writing of this report, or the decision to submit the article for publication. The authors have no conflicts of interest to disclose.