Adaptive immunity to human coronaviruses is widespread but low in magnitude
Hyon-Xhi Tan, Wen Shi Lee, Kathleen M Wragg, Christina Nelson, Robyn Esterbauer, Hannah G Kelly, Thakshila Amarasena, Robert Jones, Graham Starkey, Bao Zhong Wang, Osamu Yoshino, Thomas Tiang, Michael Lindsay Grayson, Helen Opdam, Rohit D'Costa, Angela Vago, Laura K Mackay, Claire L Gordon, Adam K Wheatley, Stephen J Kent Show all
CLINICAL & TRANSLATIONAL IMMUNOLOGY | WILEY | Published : 2021
Objectives: Endemic human coronaviruses (hCoVs) circulate worldwide but cause minimal mortality. Although seroconversion to hCoV is near ubiquitous during childhood, little is known about hCoV-specific T-cell memory in adults. Methods: We quantified CD4 T-cell and antibody responses to hCoV spike antigens in 42 SARS-CoV-2-uninfected individuals. Antigen-specific memory T cells and circulating T follicular helper (cTFH) cells were identified using an activation-induced marker assay and characterised for memory phenotype and chemokine receptor expression. Results: T-cell responses were widespread within conventional memory and cTFH compartments but did not correlate with IgG titres. SARS-CoV-2..View full abstract
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The authors thank the study participants and clinical teams for their participation in and assistance with the study. We gratefully acknowledge the generosity of the organ donor families for providing valuable tissue samples. We acknowledge the Melbourne Cytometry Program for provision of flow cytometry services. Funding for this work was provided by the Victorian Government, a Doherty Collaborative Research Award (JJ), the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (SJK), an NHMRC Program Grant APP1149990 (SJK), and philanthropic support from the Paul Ramsay Foundation (SJK and AKW). AKW is funded by an NHMRC Investigator Grant. JAJ and SJK are funded by NHMRC fellowships.