Journal article
Dehydration-induced proteomic landscape of mitochondria in chickpea reveals large-scale coordination of key biological processes
Dipak Gayen, Saurabh Gayali, Pragya Barua, Nilesh Vikram Lande, Swati Varshney, Shantanu Sengupta, Subhra Chakraborty, Niranjan Chakraborty
JOURNAL OF PROTEOMICS | ELSEVIER SCIENCE BV | Published : 2019
Abstract
Mitochondria play crucial roles in regulating multiple biological processes particularly electron transfer and energy metabolism in eukaryotic cells. Exposure to water-deficit or dehydration may affect mitochondrial function, and dehydration response may dictate cell fate decisions. iTRAQ-based quantitative proteome of a winter legume, chickpea, demonstrated the central metabolic alterations in mitochondria, presumably involved in dehydration adaptation. Three-week-old chickpea seedlings were subjected to progressive dehydration and the magnitude of dehydration-induced compensatory physiological responses was monitored in terms of physicochemical characteristics and mitochondrial architectur..
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Awarded by Department of Biotechnology (DBT), India
Awarded by Department of Science and Technology (DST), India
Funding Acknowledgements
This work was supported by grants from the Department of Biotechnology (DBT), India (BT/AGR/CG-PhaseII/01/2014) and National Institute of Plant Genome Research, New Delhi to N.C. We thank Department of Science and Technology (DST), India for SERB-National Post-Doctoral Fellowship (PDF/2016/002615) granted to D.G. and Council of Scientific & Industrial Research (CSIR), India for providing pre-doctoral fellowship to S.G. and N.V.L. We also acknowledge DBT for providing pre-doctoral fellowship to P.B. We thank Dr. J.G Kapuganti and Dr. Sonika Pandey for valuable suggestions and guidance in measurement of mitochondrial respiration parameters. We thank Mr. Jasbeer Singh for illustrations and graphical representation in the manuscript.