Journal article
A Mycoplasma gallisepticum Glycerol ABC Transporter Involved in Pathogenicity
S Mahdizadeh, Y Masukagami, CW Tseng, PF Markham, DP De Souza, B Nijagal, D Tull, L Tatarczuch, GF Browning, FM Sansom
Applied and Environmental Microbiology | AMER SOC MICROBIOLOGY | Published : 2021
DOI: 10.1128/AEM.03112-20
Abstract
MalF has been shown to be required for virulence in the important avian pathogen Mycoplasma gallisepticum. To characterize the function of MalF, predicted to be part of a putative ABC transporter, we compared metabolite profiles of a mutant with a transposon inserted in malF (MalF-deficient ST mutant 04-1; ΔmalF) with those of wild-type bacteria using gas chromatography-mass spectrometry and liquid chromatography- mass spectrometry. Of the substrates likely to be transported by an ABC transport system, glycerol was detected at significantly lower abundance in the DmalF mutant, compared to the wild type. Stable isotope labeling using [U-13C]glycerol and reverse transcription-quantitative PCR ..
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Funding Acknowledgements
S.M. was supported by a Research Training Program Scholarship provided by the University of Melbourne, Australia. This study used NCRIS-enabled Metabolomics Australia infrastructure at the University of Melbourne and funded through BioPlatforms Australia.