Identifying microRNA determinants of human myelopoiesis
Megha Rajasekhar, Ulf Schmitz, Stephane Flamant, Justin J-L Wong, Charles G Bailey, William Ritchie, Jeff Holst, John EJ Rasko
SCIENTIFIC REPORTS | NATURE PUBLISHING GROUP | Published : 2018
Myelopoiesis involves differentiation of hematopoietic stem cells to cellular populations that are restricted in their self-renewal capacity, beginning with the common myeloid progenitor (CMP) and leading to mature cells including monocytes and granulocytes. This complex process is regulated by various extracellular and intracellular signals including microRNAs (miRNAs). We characterised the miRNA profile of human CD34+CD38+ myeloid progenitor cells, and mature monocytes and granulocytes isolated from cord blood using TaqMan Low Density Arrays. We identified 19 miRNAs that increased in both cell types relative to the CMP and 27 that decreased. miR-125b and miR-10a were decreased by 10-fold a..View full abstract
Awarded by Cancer Council NSW
Awarded by NHMRC
The authors acknowledge grant funding from the Cancer Council NSW RG11-11 and NHMRC #1080530 both to JEJR. JEJR was funded by Cure the Future and an anonymous foundation. US holds a fellowship from the Cancer Institute of NSW. The authors would like to thank the Sydney Cord Blood Bank for the cord blood samples, the Centenary Institute Flow Cytometry Facility and Applied Biosystems for supplying the TLDAs used in this study.