Precise single base substitution in the shibire gene by CRISPR/Cas9-mediated homology directed repair in Bactrocera tryoni
Amanda Choo, Elisabeth Fung, Isabel Y Chen, Robert Saint, Peter Crisp, Simon W Baxter
BMC Genetics | BioMed Central | Published : 2020
Background Pest eradication using the Sterile Insect Technique (SIT) involves high-density releases of sterilized males that mate with wild females and ultimately suppress the population. Sterilized females are not required for SIT and their removal or separation from males prior to release remains challenging. In order to develop genetic sexing strains (GSS), conditional traits such as temperature sensitive lethality are required. Results Here we introduce a known Drosophila melanogaster temperature sensitive embryonic lethal mutation into Bactrocera tryoni, a serious horticultural pest in Australia. A non-synonymous point mutation in the D. melanogaster gene shibire causes embryonic letha..View full abstract
Awarded by Australian Research Council
Awarded by Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA Vienna, Austria
This project is part of the National SITplus program and has been funded by Horticulture Innovation Australia, using the research and development levy funds from the vegetable, apple and pear, citrus, strawberry, table grape, cherry and summerfruit industries, with co-investment from South Australian Research and Development Institute (SARDI) and Primary Industries and Regions South Australia (PIRSA), and funds from the Australian Government. SWB is supported by the Australian Research Council (FT140101303). The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. Publication costs are funded by the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, IAEA (CRP No.: D4.20.16) Vienna, Austria.